Journal: Cancer prevention research (Philadelphia, Pa.)
Article Title: Precision cut lung slices as a preclinical model for non-small cell lung cancer chemoprevention
doi: 10.1158/1940-6207.CAPR-23-0004
Figure Lengend Snippet: (A) Schematic of the PCLS generation process. (Diagram created with Biorender.com .) (B) H&E of frozen wild type mouse PCLS at day 1 and day 7 after treatment with Iloprost (3.6μg/ml), vehicle, or empty control. (C) A Presto Blue assay measured viability of wild type mouse PCLS at day 1, 3, and 7 during treatment with vehicle, iloprost (3.6μg/ml), or empty (n=5). Data is shown relative to day 1 (dotted line). (D) PPARγ signaling was measured by PPRE luciferase assay in culture media from PCLS with vehicle or iloprost (3.6ug/ml) at day 6. Data is shown relative to WT control and the assay was conducted in triplicate (n=3). (E) E-cadherin expression in tissue from WT or Fzd9 −/− mice with control or iloprost (3.6μg/ml) was measured by qPCR. Assays were conducted in triplicate, data is normalized to RPS18 and shown relative to WT control (n=3). WT, wild type; Fzd9 −/− . Frizzled 9 knockout; ILO, iloprost. Error bars are SEM. Significance was measured by one-way ANOVA with Tukey post-hoc analysis. *p≤0.05
Article Snippet: Animals and PCLS: Frizzled 9 knockout mice ( Fzd9 −/− ) on an FVB/N (RRID:MGI:3528175) background were developed by the Regional Mouse Genetics Core Facility at National Jewish Health and the University of Colorado as previously described. ( 19 ) Wild type female FVB/N mice (eight weeks old, Charles River Laboratories) and female Fzd9 −/− mice were housed in a pathogen-free facility in the Rocky Mountain Regional Veterans Medical Center (RMRVAMC) Veterinary Medical Unit (VMU).
Techniques: Control, Luciferase, Expressing, Knock-Out